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Human Lens Epithelial Cell Line, #HLE-B3

Name: Human Lens Epithelial Cell Line, #HLE-B3 - Celltech Discovery Solutions - The Cell Culture Company
SKU: CDS10552
Price: 396.00 USD
Availability: InStock

$396.00

Human Vaginal Epithelial Cell Line, #VK2/E6E7 is an established non-tumor cell model for in vitro research.
Epithelial-cell models help explore how tissue surfaces maintain their organization and respond to stress.
They are relevant to studies of cell renewal, barrier-associated processes and local inflammation.

Pack Size: 1 x 1 million cells

📄 Validation Guide

📄 Datasheet

In stock

SKU:CDS10552

Human Lens Epithelial Cell Line, #HLE-B3 is an established non-tumor cell model for in vitro research. It is suitable for studies of cell physiology, growth, signaling, toxicity, drug response, biomarker analysis and assay development under controlled culture conditions. The cell line provides a reproducible system for basic and translational cell biology. For research use only.

Application:
1. Cancer Biology Research
Mechanistic Studies
Gene Function Studies
Signal Transduction

2. Drug Discovery and Development
Screening of Anticancer Compounds
IC50/EC50 Studies
Mechanism of Action
Combination Therapies

3. Assay Development
ELISA and Immunoassays
Flow Cytometry Assays
Reporter Gene Assays

4. Omics and Biomarker Research
Transcriptomics / Genomics
Proteomics
Metabolomics

5. Tumor Heterogeneity and Resistance Mechanisms
Clonal Selection
Acquired Resistance Models

6. Personalized Medicine and Precision Oncology
Patient-Derived Cell Lines
Companion Diagnostics

7. Immuno-oncology
Co-culture with Immune Cells
Checkpoint Inhibitor Testing
Antibody-Dependent Cytotoxicity (ADCC)

8. Vaccine Development
Neoantigen Discovery
Antigen Presentation Studies

Pack Size	1 x 1 million cells
Application	1. Cancer Biology Research Mechanistic Studies Gene Function Studies Signal Transduction 2. Drug Discovery and Development Screening of Anticancer Compounds IC50/EC50 Studies Mechanism of Action Combination Therapies 3. Assay Development ELISA and Immunoassays Flow Cytometry Assays Reporter Gene Assays 4. Omics and Biomarker Research Transcriptomics / Genomics Proteomics Metabolomics 5. Tumor Heterogeneity and Resistance Mechanisms Clonal Selection Acquired Resistance Models 6. Personalized Medicine and Precision Oncology Patient-Derived Cell Lines Companion Diagnostics 7. Immuno-oncology Co-culture with Immune Cells Checkpoint Inhibitor Testing Antibody-Dependent Cytotoxicity (ADCC) 8. Vaccine Development Neoantigen Discovery Antigen Presentation Studies
Alternate Name 1	CRL-11421 Cells
Alternate Name 2	HLE-B3 Cells
Primary Tissue	Lens Epithelial
Type of Cells	Mixed Adherent/Suspension
Biosafety Level	BSL-2
Culture Medium	MEM + 20% FBS + 1% Pen/Strep
Freezing Medium	55% Complete Culture Medium + 40% FBS + 5% DMSO
Cryopreservation	please contact us immediately., Upon Receipt of Cells 1 mL cryovials are packed in dry ice for transportation. After receipt
Culture Conditions	2-3 mL for T75 flask), 5) Place in a 37°C incubator for digestion for 1-2 minutes (difficult-to-digest cells can be properly prolonged for digestion time), 6) Observe the cell digestion under a microscope. If most of the cells become round and fall off, and add 3-4 ml of culture medium containing 10% FBS to terminate digestion. 7) Gently mix and aspirate, and perform subsequent passages at a ratio of 1:2~1:5 as per actual conditions., and rinse the cells 1-2 times with PBS without calcium and magnesium ions. 4) Add 0.25% (w/v) trypsin-0.53 mM EDTA to the culture flask (1-2 mL for T25 flask, centrifuge at 1000rpm for 3-5min, quickly take it back to the operating table, Recovery of Frozen Cells: For Suspension Cell passaging, subculture can be performed. For Adherent Cell passaging, tap the culture flask a few times
Tumorigenic / Normal	Normal Cells
Target / Construct	N/A
Assay / Validation Method	Flow cytometry marker characterization
Sterility	Bacteria: Negative Yeast: Negative Mycoplasma: Negative
Pathogens	HIV: Negative Hepatitis B: Negative
Usage Terms	For Research Use Only. Not for diagnostics or human use.
Shelf Life	12 months at time of shipping
Shipping	Shipped on Dry Ice at -20ºC
Storage	Store at -176ºC.
Disclaimer	The data indicated herein are as indicated and validated in our laboratory. As these cell lines are of human origin, they carry a risk of latent human viruses (e.g., Epstein–Barr virus, hepatitis B). Therefore they should be handled accordingly with precaution. These reagents are for research use only and not for in-vitro diagnostics or human use.