Immortalized Microvascular Endothelial Cell Line Of The Human Brain, #HCMEC/D3 is an established non-tumor cell model for in vitro research. It is suitable for studies of cell physiology, growth, signaling, toxicity, drug response, biomarker analysis and assay development under controlled culture conditions. The cell line provides a reproducible system for basic and translational cell biology. For research use only.
Immortalized Microvascular Endothelial Cell Line Of The Human Brain, #HCMEC/D3
$550.00
Immortalized Microvascular Endothelial Cell Line Of The Human Brain, #HCMEC/D3 is an immortalized cell model for sustained in vitro investigation of the named cell type.
Vascular-cell models help researchers investigate the lining that separates blood from surrounding tissues.
They are relevant to studies of barrier regulation, inflammatory responses and vessel-associated biology.
Pack Size: 1 x 1 million cells
Validation Guide
In stock
| Pack Size | 1 x 1 million cells |
|---|---|
| Application | 1. Cancer Biology Research Mechanistic Studies Gene Function Studies Signal Transduction 2. Drug Discovery and Development Screening of Anticancer Compounds IC50/EC50 Studies Mechanism of Action Combination Therapies 3. Assay Development ELISA and Immunoassays Flow Cytometry Assays Reporter Gene Assays 4. Omics and Biomarker Research Transcriptomics / Genomics Proteomics Metabolomics 5. Tumor Heterogeneity and Resistance Mechanisms Clonal Selection Acquired Resistance Models 6. Personalized Medicine and Precision Oncology Patient-Derived Cell Lines Companion Diagnostics 7. Immuno-oncology Co-culture with Immune Cells Checkpoint Inhibitor Testing Antibody-Dependent Cytotoxicity (ADCC) 8. Vaccine Development Neoantigen Discovery Antigen Presentation Studies |
| Alternate Name 1 | CVCL_B254 Cells |
| Alternate Name 2 | HCMEC/D3 Cells |
| Primary Tissue | Brain |
| Type of Cells | Adherent Cells |
| Biosafety Level | BSL-2 |
| Culture Medium | Endothelial cell culture system (CTDS) |
| Freezing Medium | 55% Complete Culture Medium + 40% FBS + 5% DMSO |
| Cryopreservation | please contact us immediately., Upon Receipt of Cells 1 mL cryovials are packed in dry ice for transportation. After receipt |
| Culture Conditions | 2-3 mL for T75 flask), 5) Place in a 37°C incubator for digestion for 1-2 minutes (difficult-to-digest cells can be properly prolonged for digestion time), 6) Observe the cell digestion under a microscope. If most of the cells become round and fall off, and add 3-4 ml of culture medium containing 10% FBS to terminate digestion. 7) Gently mix and aspirate, and perform subsequent passages at a ratio of 1:2~1:5 as per actual conditions., and rinse the cells 1-2 times with PBS without calcium and magnesium ions. 4) Add 0.25% (w/v) trypsin-0.53 mM EDTA to the culture flask (1-2 mL for T25 flask, centrifuge at 1000rpm for 3-5min, quickly take it back to the operating table, Recovery of Frozen Cells: For Suspension Cell passaging, subculture can be performed. For Adherent Cell passaging, tap the culture flask a few times |
| Tumorigenic / Normal | Normal Cells |
| Target / Construct | N/A |
| Assay / Validation Method | Flow cytometry marker characterization |
| Sterility | Bacteria: Negative Yeast: Negative Mycoplasma: Negative |
| Pathogens | HIV: Negative Hepatitis B: Negative |
| Usage Terms | For Research Use Only. Not for diagnostics or human use. |
| Shelf Life | 12 months at time of shipping |
| Shipping | Shipped on Dry Ice at -20ºC |
| Storage | Store at -176ºC. |
| Disclaimer | The data indicated herein are as indicated and validated in our laboratory. As these cell lines are of human origin, they carry a risk of latent human viruses (e.g., Epstein–Barr virus, hepatitis B). Therefore they should be handled accordingly with precaution. These reagents are for research use only and not for in-vitro diagnostics or human use. |
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