Primary Canine Adult Epidermal Keratinocytes (AEK) is a primary cell product from canine Adult Epidermal tissue for physiologically relevant in vitro studies. The product is particularly relevant to Keratinocytes and Skeletal, articular and cutaneous systems research. It can support research in cell physiology, signaling, metabolism, toxicology, drug response and cell-based assay development where a primary-cell model is preferred. Culture and passage conditions should follow the product-specific protocol. For research use only.
Primary Canine Adult Epidermal Keratinocytes (AEK)
$1,280.00
Primary Canine Adult Epidermal Keratinocytes (AEK) provides a primary-cell model for investigating the biology of the specified cell population.
Epithelial-cell models help explore how tissue surfaces maintain their organization and respond to stress.
Applications include cell-function studies, tissue-response research and evaluation of biological or chemical interventions under cell-specific culture conditions.
Pack Size: 1 x 0.5 million cells
Validation Guide
In stock
| Pack Size | 1 x 0.5 million cells |
|---|---|
| Application | Keratinocytes;Skeletal, articular and cutaneous systems |
| Alternate Name 1 | Adult Epidermal Keratinocyte Cells |
| Alternate Name 2 | Adult Epidermal Keratinocytes (AEK) |
| Primary Tissue | Adult Epidermal tissue |
| Type of Cells | Adherent Cells |
| Biosafety Level | BSL-2 |
| Culture Medium | 5% carbon dioxide, DMEM/F12 + 10% FBS + 1% Keratinocyte Growth Supplement + 1% Pen/Step. Temperature: 37°C. Incubation Condition: 95% air |
| Freezing Medium | 55% Complete Culture Medium + 40% FBS + 5% DMSO |
| Cryopreservation | please contact us immediately., Upon Receipt of Cells 1 mL cryovials are packed in dry ice for transportation. After receipt |
| Culture Conditions | 2-3 mL for T75 flask), 5) Place in a 37°C incubator for digestion for 1-2 minutes (difficult-to-digest cells can be properly prolonged for digestion time), 6) Observe the cell digestion under a microscope. If most of the cells become round and fall off, and add 3-4 ml of culture medium containing 10% FBS to terminate digestion. 7) Gently mix and aspirate, and perform subsequent passages at a ratio of 1:2~1:5 as per actual conditions., and rinse the cells 1-2 times with PBS without calcium and magnesium ions. 4) Add 0.25% (w/v) trypsin-0.53 mM EDTA to the culture flask (1-2 mL for T25 flask, centrifuge at 1000rpm for 3-5min, quickly take it back to the operating table, Recovery of Frozen Cells: For Suspension Cell passaging, subculture can be performed. For Adherent Cell passaging, tap the culture flask a few times |
| Tumorigenic / Normal | Normal Cells |
| Target / Construct | N/A |
| Assay / Validation Method | Flow cytometry marker characterization |
| Sterility | Bacteria: Negative Yeast: Negative Mycoplasma: Negative |
| Pathogens | HIV: Negative Hepatitis B: Negative |
| Usage Terms | For Research Use Only. Not for diagnostics or human use. |
| Shelf Life | 12 months at time of shipping |
| Shipping | Shipped on Dry Ice at -20ºC |
| Storage | Store at -176ºC. |
| Disclaimer | The data indicated herein are as indicated and validated in our laboratory. As these cell lines are of human origin, they carry a risk of latent human viruses (e.g., Epstein–Barr virus, hepatitis B). Therefore they should be handled accordingly with precaution. These reagents are for research use only and not for in-vitro diagnostics or human use. |
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