Primary Mouse Auricular Chondrocytes (ACC) is a primary cell product from mouse Auricular Cartilage for physiologically relevant in vitro studies. The product is particularly relevant to Ophthalmology & Otorhinolaryngology and Chondrocytes research. It can support research in cell physiology, signaling, metabolism, toxicology, drug response and cell-based assay development where a primary-cell model is preferred. Culture and passage conditions should follow the product-specific protocol. For research use only.
Primary Mouse Auricular Chondrocytes (ACC)
$860.00
Primary Mouse Auricular Chondrocytes (ACC) provides a primary-cell model for investigating the biology of the specified cell population.
Skeletal-tissue cell models support research into matrix production and tissue maintenance.
Applications include cell-function studies, tissue-response research and evaluation of biological or chemical interventions under cell-specific culture conditions.
Pack Size: 1 x 0.5 million cells
Validation Guide
In stock
| Pack Size | 1 x 0.5 million cells |
|---|---|
| Application | Ophthalmology & Otorhinolaryngology;Chondrocytes;Eye, ear, nose, throat and oral systems |
| Alternate Name 2 | Auricular Chondrocytes (ACC) |
| Primary Tissue | Auricular Cartilage |
| Type of Cells | Adherent Cells |
| Biosafety Level | BSL-2 |
| Culture Medium | 5% carbon dioxide, DMEM/F12 + 10% FBS + 1% Chondrocyte Growth Supplement + 1% Pen/Step. Temperature: 37°C Incubation Condition: 95% air |
| Freezing Medium | 55% Complete Culture Medium + 40% FBS + 5% DMSO |
| Cryopreservation | please contact us immediately., Upon Receipt of Cells 1 mL cryovials are packed in dry ice for transportation. After receipt |
| Culture Conditions | 2-3 mL for T75 flask), 5) Place in a 37°C incubator for digestion for 1-2 minutes (difficult-to-digest cells can be properly prolonged for digestion time), 6) Observe the cell digestion under a microscope. If most of the cells become round and fall off, and add 3-4 ml of culture medium containing 10% FBS to terminate digestion. 7) Gently mix and aspirate, and perform subsequent passages at a ratio of 1:2~1:5 as per actual conditions., and rinse the cells 1-2 times with PBS without calcium and magnesium ions. 4) Add 0.25% (w/v) trypsin-0.53 mM EDTA to the culture flask (1-2 mL for T25 flask, centrifuge at 1000rpm for 3-5min, quickly take it back to the operating table, Recovery of Frozen Cells: For Suspension Cell passaging, subculture can be performed. For Adherent Cell passaging, tap the culture flask a few times |
| Tumorigenic / Normal | Normal Cells |
| Target / Construct | N/A |
| Assay / Validation Method | Flow cytometry marker characterization |
| Sterility | Bacteria: Negative Yeast: Negative Mycoplasma: Negative |
| Pathogens | HIV: Negative Hepatitis B: Negative |
| Usage Terms | For Research Use Only. Not for diagnostics or human use. |
| Shelf Life | 12 months at time of shipping |
| Shipping | Shipped on Dry Ice at -20ºC |
| Storage | Store at -176ºC. |
| Disclaimer | The data indicated herein are as indicated and validated in our laboratory. As these cell lines are of human origin, they carry a risk of latent human viruses (e.g., Epstein–Barr virus, hepatitis B). Therefore they should be handled accordingly with precaution. These reagents are for research use only and not for in-vitro diagnostics or human use. |
Reviews
There are no reviews yet.