Human Renal Cell Adenocarcinoma Cell Line, #ACHN is an established tumor-derived cell line for in vitro oncology research. It is suitable for studies of tumor cell biology, proliferation and viability, signaling, biomarkers, drug response, cytotoxicity and cell-based assay development. The model can be incorporated into screening and mechanistic workflows under validated culture conditions. For research use only.
Human Renal Cell Adenocarcinoma Cell Line, #ACHN
$330.00
Human Renal Cell Adenocarcinoma Cell Line, #ACHN is an established tumor-derived cell line for in vitro oncology research.
Urinary-system cancer models support studies of tumor-cell survival and responses to candidate treatments.
Applications include studies of proliferation, survival signaling, cytotoxicity and comparative responses to experimental treatments.
Pack Size: 1 x 1 million cells
Validation Guide
In stock
| Pack Size | 1 x 1 million cells |
|---|---|
| Application | 1. Cancer Biology Research Mechanistic Studies Gene Function Studies Signal Transduction 2. Drug Discovery and Development Screening of Anticancer Compounds IC50/EC50 Studies Mechanism of Action Combination Therapies 3. Assay Development ELISA and Immunoassays Flow Cytometry Assays Reporter Gene Assays 4. Omics and Biomarker Research Transcriptomics / Genomics Proteomics Metabolomics 5. Tumor Heterogeneity and Resistance Mechanisms Clonal Selection Acquired Resistance Models 6. Personalized Medicine and Precision Oncology Patient-Derived Cell Lines Companion Diagnostics 7. Immuno-oncology Co-culture with Immune Cells Checkpoint Inhibitor Testing Antibody-Dependent Cytotoxicity (ADCC) 8. Vaccine Development Neoantigen Discovery Antigen Presentation Studies |
| Alternate Name 1 | CVCL_1067 Cells |
| Alternate Name 2 | ACHN-1/ACHN1 Cells |
| Primary Tissue | Renal Cell |
| Type of Cells | Adherent Cells |
| Biosafety Level | BSL-2 |
| Culture Medium | MEM + 10% FBS + 1% Sodium Pyruvate + 1% L-Glutamine + 1% Pen/Strep |
| Freezing Medium | 55% Complete Culture Medium + 40% FBS + 5% DMSO |
| Cryopreservation | please contact us immediately., Upon Receipt of Cells 1 mL cryovials are packed in dry ice for transportation. After receipt |
| Culture Conditions | 2-3 mL for T75 flask), 5) Place in a 37°C incubator for digestion for 1-2 minutes (difficult-to-digest cells can be properly prolonged for digestion time), 6) Observe the cell digestion under a microscope. If most of the cells become round and fall off, and add 3-4 ml of culture medium containing 10% FBS to terminate digestion. 7) Gently mix and aspirate, and perform subsequent passages at a ratio of 1:2~1:5 as per actual conditions., and rinse the cells 1-2 times with PBS without calcium and magnesium ions. 4) Add 0.25% (w/v) trypsin-0.53 mM EDTA to the culture flask (1-2 mL for T25 flask, centrifuge at 1000rpm for 3-5min, quickly take it back to the operating table, Recovery of Frozen Cells: For Suspension Cell passaging, subculture can be performed. For Adherent Cell passaging, tap the culture flask a few times |
| Tumorigenic / Normal | Tumor Cells |
| Target / Construct | N/A |
| Assay / Validation Method | Flow cytometry marker characterization |
| Sterility | Bacteria: Negative Yeast: Negative Mycoplasma: Negative |
| Pathogens | HIV: Negative Hepatitis B: Negative |
| Usage Terms | For Research Use Only. Not for diagnostics or human use. |
| Shelf Life | 12 months at time of shipping |
| Shipping | Shipped on Dry Ice at -20ºC |
| Storage | Store at -176ºC. |
| Disclaimer | The data indicated herein are as indicated and validated in our laboratory. As these cell lines are of human origin, they carry a risk of latent human viruses (e.g., Epstein–Barr virus, hepatitis B). Therefore they should be handled accordingly with precaution. These reagents are for research use only and not for in-vitro diagnostics or human use. |
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